dh5α competent cells (Sangon Biotech)

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Sangon Biotech dh5α competent cells
Dh5α Competent Cells, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dh5%CE%B1+competent+cells/bl21+coli+e/pmc13202259-17-0-4
Average 86 stars, based on 1 article reviews

dh5α competent cells - by Bioz Stars, 2026-10

86/100 stars

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Ligation:

Article Title: Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone.
Article Snippet: .. The ligation product was transformed into DH5α competent cells (Sangon Biotech, Shanghai) and plated on LB agar plates containing 50 mg/L kanamycin, followed by incubation at 37°C for 12 h. Colony PCR and restriction enzyme digestion analysis were performed using the pCB301- F/R primers (Table 1). ..

Article Title: Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone
Article Snippet: .. The ligation product was transformed into DH5α competent cells (Sangon Biotech, Shanghai) and plated on LB agar plates containing 50 mg/L kanamycin, followed by incubation at 37°C for 12 h. Colony PCR and restriction enzyme digestion analysis were performed using the pCB301‐F/R primers (Table ). ..

Transformation Assay:

Article Title: Identification of genes controlling grain size in barley through RNA-seq and weighted gene co-expression network analysis.
Article Snippet: .. The constructs were then transformed into DH5α competent cells (Sangon Biotech, Shanghai, China). .. Three single positive colonies were selected and sent for sequencing at TsingKe Biotechnology Co., Ltd. (TsingKe, Xi’an, China).

Article Title: Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone.
Article Snippet: The purified fragments were ligated into pMD19- T vectors (TaKaRa). .. The recombinant plasmid was transformed into DH5α competent cells (Sangon Biotech, Shanghai). ..

Incubation:

Polymerase Chain Reaction:

Plasmid Preparation:

Article Title: Construction of a DNA Methylation Map of Argali Hybrid Sheep During Mo Infection
Article Snippet: .. The pUCm-T vector and DH5α competent cells were obtained from Sangon Biotech (Shanghai, China). .. Tissue DNA was extracted using the Vazyme DNA Isolation Mini Kit (Nanjing, China; Cat# DC-103-01).

Construct:

other:

Article Title: Establishment and field validation of a rapid on-site recombinase polymerase amplification–lateral flow assay for BRSV and BVDV
Article Snippet: DH5α competent cells were purchased from Sangon Biotech (Shanghai) Co., Ltd.

Review